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Cas-Designer
A bulge-allowed quick guide-RNA designer for CRISPR/Cas derived RGENs.
Cas-Designer provides all possible RGEN targets in the given input sequence (e.g. exon), with the useful information including potential off-target numbers within 2nt mismatches and optional 3nt bulgeviaCas-OFFinder, and out-of-frame scores viaMicrohomology-predictor. If you want to search with more mismatch numbers or bigger bulge size, then you can download and use a standalone edition of Cas-Designer.
Citation info: For Microhomology-based target selection:
Submit a new job, or download a standalone edition of Cas-Designer here.
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